Organic Acid Test: Part 2, Intestinal Microbiomes

Please note: The content of this post is my own unless the technical terms are too hard to explain, then the content is copy and paste. I am not a medical professional and thus the post is my point of view. But the content is science-based and credible. Just because it is a new science, does not necessarily make it quackery.

Results part 1 – Intestinal Microbiome

The test was done by my son’s specialist through North West University.

Urine organic acids: Micobiome markers

2,5-Furandicarboxylic acid 5.17 < 12 mmol/mol creat
2-Hydroxyphenylacetic acid 0.68 < 20 mmol/mol creat
3,4-Dihydroxyphenylpropionic acid 0.11 < 0.35 mmol/mol creat
3,5-Dihydroxyphenylpropionoic acid (DHPPA) 0.04 < 0.38 mmol/mol creat
3-Hydroxyphenyl-3-hydroxypropionic acid  (HPHPA) 1.81 < 91.5 mmol/mol creat
3-Indoleacetic acid 4.28 < 12.3 mmol/mol creat
3-Oxoglutaric acid/3-Ketoglutaric acid BDL < 0.46 mmol/mol creat
4-Hydroxybenzoic acid 8.57 H < 3.6 mmol/mol creat
4-Hydroxyhippuric acid 7.76 < 14 mmol/mol creat
4-Hydroxyphenylacetic acid 21.93 9.7 – 187.4 mmol/mol creat
5-Hydroxymethyl-2-furoic acid (Sumiki’s acid) 8.09 H < 1.7 mmol/mol creat
Arabinose 4.28 < 19.4 mmol/mol creat

The Microbiome makers look for:

  1. Yeast (Candida)
  2. Mould (Aspergillus)
  3. Fungus
  4. Bacterial
  5. Clostridia
Yeast

Candida albicans is the most common form of yeast in the body. Candida can build up toxins that contribute to fibromyalgia, migraines, chronic fatigue syndrome, brain fog, depression, and other symptoms.

In the Organic Acid test, Arabinose is normally an indicator for Candida. Tartaric Acid and 3-Oxoglutaric are also indicators of a candida infection.   When Candida becomes invasive, it attaches to the intestinal wall causing leaky gut. This can result in food sensitivities, nutrient malabsorption, and intestinal disorders.

(As from Great Plains Clinical break down sheet:

Arabinose  Produced by the action of Candida hyaluronidase on the intercellular cement hyaluronic acid.  Oxidation of the hyaluronic acid breakdown products produces tartaric acid and arabinose.  Since arabinose is also a major sugar in apples, grapes, and pears, these fruits and their products must be avoided 24 hours prior to urine collection to avoid interference.  Antifungal treatment and high-potency multi-strain probiotics may help rebalance GI flora.

Tartaric Acid Produced by the action of Candida hyaluronidase on the intercellular cement hyaluronic acid. Oxidation of the hyaluronic acid breakdown products produces tartaric acid and arabinose.  Since grapes, grape juice, and dried grapes (raisins) contain tartaric acid, these foods must be avoided 24 hours prior to urine collection to avoid interference.  Antifungal treatment and high-potency multi-strain probiotics may help rebalance GI flora. 

3-Oxoglutaric Acid Indicates a possible yeast overgrowth in the GI tract.  High-potency multi-strain probiotics may help rebalance GI flora.)

Clostridia

The toxins released by Clostridia metabolites can inhibit key neurotransmitters in the brain. Clostridia is associated with gastrointestinal disorders including irritable bowel syndrome, Crohn’s disease, and ulcerative colitis.

The most common clostridial infection is gastroenteritis (Clostridium perfringens food poisoning), a usually mild infection that typically resolves on its own.

If makers 4-Hydroxyphenylacetic, HPHPA and 4-Cresol is elevated then you have a Clostridia infection.

These markers can inhibit the enzyme dopamine beta-hydroxylase which can elevate dopamine and cause neurotransmitter imbalances. Both HPHPA and 4-Cresol are said to be higher in Autistic, people with ADHD and other psychiatric disorders.

It is said that 4-Cresol feeds on Tyrosine.

(As from the Great Plains Lab clinical breakdown sheet:

4-Hydroxyphenylacetic A tyrosine metabolic product of GI bacteria.  Elevated levels are associated with bacterial overgrowth or small bowel disease.  May also indicate celiac disease.

HPHPA An abnormal phenylalanine metabolite produced by gastrointestinal bacteria of Clostridia species, including sporogenes, botulinum, caloritolerans, mangenoti, ghoni, bifermentans, difficile, and   sordellii.  Negative stool tests for C.difficile do not rule out the presence of other Clostridia species.  This metabolite may is frequently associated with behavioural and neurological abnormalities that resolve after antimicrobial therapy.  In many cases, Clostridia overgrowth can be controlled by supplementation with 30 billion cells per day of Lactobacillus rhamnosus GG (Culturelle) and/or 2-6 billion cfu’s of Saccharomyces boulardii.   

4-Cresol Indicates a possible overgrowth of intestinal bacteria that are specific p-cresol producers including 4-Cresol is a phenolic product poorly metabolized in children with autism.  High-potency multi-strain probiotics may help rebalance GI flora.)

Fungus

The Tricarballyllic acid maker is an indicator of a fungal infection. Overgrowths of yeast and fungi poison the mitochondria and contribute to mitochondrial dysfunction, stress hormone imbalances, and nutritional malabsorption.

(As from the Great Plains Lab breakdown sheet:

Tricarballyllic Acid A chemical by-product released from fumonisins during passage through the gastrointestinal tract. Fumonisins are fungal toxins produced primarily by F. verticillioides.  Elevated levels can be caused by the intake of corn or corn-based food contaminated with fumonisins. )

Good Bacteria

The biomarker DHPPA shows how much good bacteria you have in your gut.

(As from the Great Plains Labs breakdown Sheet:

DHPPA  DHPPA in urine indicates intake of chlorogenic acid, a common substance in beverages and many fruits and vegetables.  Harmless or beneficial bacteria such as Lactobacilli, Bifidobacteria, and E. coli increase the breakdown of chlorogenic acid to DHPPA so high values are mainly associated with increased amounts of these species in the GI tract.)

The only two markers left is the Mold and the Bacterial, those were the ones that were most elevated on Alex’s test.

Mold

The Aspergillus mold is the most common one found in the gut. The presence of Aspergillus indicates mold from food and water.

The markers for mold is 5-Hydroxymethyl-2-Furoic (Sumiki’s acid), Furan-2.5-dicarboxylic acid and N-2-Furancarbonylglycine:

5-Hydroxymethyl-2-Furoic (Sumiki’s acid)            8.9 H / <1.7

These markers are important because Aspergillus produces a specific mycotoxin called aflatoxin which is a carcinogen. These mycotoxins are also damaging to the kidneys and liver. The presence of mold can suppress immune function in the gut increasing susceptibility to other microbial overgrowths.

(As per the Great Plains Labs breakdown sheet:

5-Hydroxymethyl-2-Furoic (Sumiki’s acid) A metabolite produced by Aspergillus and possibly other fungal species in the GI tract.  Prescription or natural antifungals, along with high potency multi-strain probiotics, may reduce overgrowth levels. 

Furan-2.5-dicarboxylic acid A metabolite produced by Aspergillus and possibly other fungal species in the GI tract.  Prescription or natural antifungals, along with high potency multi-strain probiotics, may reduce overgrowth levels. 

N-2-Furancarbonylglycine A metabolite natural antifungals, along with high potency multi-strain probiotics, may reduce overgrowth. produced by Aspergillus and possibly other fungal species in the GI tract.)

You can read an interesting article about mold from Dr Jockers – Mold Exposure: Signs of Mold growth & health risks

Bacterial

2-Hydroxyphenylacetic Acid, 4 Hydroxybenzoic Acid, and 4-Hydroxyhippuric Acid and Hippuric acid are indicators of bacterial overgrowth in your gut and intestines.

4-Hydroxybenzoic acid                                               8.57 H / < 3.6

Elevations in these markers are generally seen with gut dysbiosis and bacterial imbalances. 4-Hydrobenzoic acid is also an indication of eating too many jams and pie fillings containing paraben preservatives.

Parabens are derived from para-hydroxybenzoic acid (PHBA) that occurs naturally in many fruits and vegetables, such as cucumbers, cherries, carrots, blueberries and onions. PHBA also is naturally formed in the human body by the breakdown of some amino acids.

For more about Parabens, click here.

(As per the Great Plains Labs breakdown sheet:

2-Hydroxyphenylacetic Acid Elevated 2-hydroxyphenylacetic acid is associated with intestinal bacteria overgrowth.  High-potency multi-strain probiotics may help rebalance GI flora. 

4 Hydroxybenzoic Acid A marker for intestinal dysbiosis. The use of probiotics and the exclusion of paraben-containing foods is the first treatment consideration.

4-Hydroxyhippuric Acid A glycine conjugate of 4-hydroxybenzoic acid, a metabolite of paraben preservatives.  May be elevated after exposure to paraben antimicrobials in certain foods and cosmetics.  Intake of fruits containing polyphenols rich in anthocyanins, flavonols, and hydroxycinnamates may increase this compound in the urine.  Avoid exposure to parabens.   

Hippuric acid A bacterial product of phenylalanine metabolism.  Most hippuric acid in urine is derived from the microbial breakdown of chlorogenic acid, a common substance found in beverages and in many fruits and vegetables.  Higher levels indicate GI bacterial overgrowth that can be reduced with natural antibacterial agents and/or high-potency multi-strain probiotics.)

Labs comments: The microbial metabolic profile is suggestive of gut dysbiosis, primarily of yeast/fungal origin. 

Dr Lindenberg’s comments: Mixed dysbiosis (imbalance) – bacterial and fungal: start treatment with Rifaximin and Fluzol and good probiotics

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